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AgFNS overexpression increase apigenin and decrease anthocyanins in petioles of transgenic celery.

Guo-Fei Tan1, Jing Ma1, Xin-Yue Zhang1

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Summary
This summary is machine-generated.

Overexpressing the AgFNS gene in purple celery increased apigenin but decreased anthocyanin content. This study clarifies the AgFNS gene

Keywords:
AgFNSAnthocyaninsApigeninCeleryPetioleTransgene

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Area of Science:

  • Plant biochemistry
  • Metabolic engineering
  • Molecular biology

Background:

  • Apigenin and anthocyanin biosynthesis share precursors in plants.
  • Flavone synthase (FNS) is key in converting naringenin to apigenin.
  • Purple celery accumulates apigenin, but the role of AgFNS is unclear.

Purpose of the Study:

  • To clone and overexpress the AgFNS gene in purple celery.
  • To investigate the impact of AgFNS overexpression on apigenin and anthocyanin levels.
  • To elucidate the function of AgFNS in celery biosynthesis pathways.

Main Methods:

  • AgFNS gene cloning from purple celery ('Nanxuan liuhe purple celery').
  • AgFNS gene overexpression in transgenic purple celery.
  • Quantification of apigenin and anthocyanin content.
  • Analysis of gene transcript levels involved in biosynthesis pathways.

Main Results:

  • The AgFNS gene is 1068bp, encoding 355 amino acids; the protein is FSN I type.
  • AgFNS transgenic celery showed increased apigenin content in petioles.
  • Anthocyanin content decreased in petioles of AgFNS transgenic celery compared to wild-type.
  • Upregulation of early pathway genes (AgPAL, AgC4H, AgCHS, AgCHI) and downregulation of later genes (AgF3H, AgF3'H, AgDFR, AgANS, Ag3GT) were observed.

Conclusions:

  • AgFNS gene overexpression significantly alters apigenin and anthocyanin accumulation in purple celery.
  • This study provides foundational knowledge on AgFNS gene function in celery's dual biosynthesis pathways.
  • Results suggest a complex regulatory role of AgFNS in balancing apigenin and anthocyanin production.