Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cloning and sequencing of papain-encoding cDNA.

L W Cohen, V M Coghlan, L C Dihel

    Gene
    |January 1, 1986
    PubMed
    Summary

    Researchers sequenced the papain gene from papaya fruit, identifying several nucleotide differences from the expected sequence. This suggests papain is synthesized as an inactive precursor, or zymogen.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of home-based light treatment on persons with dementia and their caregivers.

    Lighting research & technology (London, England : 2001)·2015
    Same author

    AQP2 is a substrate for endogenous PP2B activity within an inner medullary AKAP-signaling complex.

    American journal of physiology. Renal physiology·2001
    Same author

    A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase.

    The Journal of biological chemistry·2001
    Same author

    Antitumor efficacy, pharmacokinetics, and biodistribution of NX 211: a low-clearance liposomal formulation of lurtotecan.

    Clinical cancer research : an official journal of the American Association for Cancer Research·2000
    Same author

    Overlay, ligand blotting, and band-shift techniques to study kinase anchoring.

    Methods in molecular biology (Clifton, N.J.)·1998
    Same author

    Identification of cGMP-dependent protein kinase anchoring proteins (GKAPs).

    Biochemical and biophysical research communications·1998

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Plant Science

    Background:

    • Papain is a cysteine protease found in Carica papaya fruit.
    • Understanding papain's genetic sequence is crucial for its biotechnological applications.

    Purpose of the Study:

    • To determine the nucleotide sequence of papain from Carica papaya fruit.
    • To identify any discrepancies between the nucleotide sequence and the known amino acid sequence of papain.

    Main Methods:

    • Messenger RNA (mRNA) was extracted from papaya fruit.
    • mRNA was converted to complementary DNA (cDNA) and cloned into plasmid pBR322.
    • Subclones of the papain gene fragment were sequenced.

    Main Results:

    • The sequenced nucleotide fragment was approximately 1.4 kb.
    • Several nucleotide differences were observed compared to the expected sequence for papain.
    • These differences included codon changes at specific amino acid positions and an altered amino acid order.
    • Analysis suggested papain is synthesized with a 133-amino acid prosegment, indicating zymogen formation.
    • An unusual (AT)9AGAA sequence was found near the stop codon.

    Conclusions:

    • The study provides the nucleotide sequence of papain from Carica papaya.
    • The identified sequence variations offer insights into papain biosynthesis and regulation.
    • Papain is likely synthesized as an inactive zymogen, requiring post-translational modification for activation.

    Related Experiment Videos