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Updated: Feb 24, 2026

Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles
Published on: August 28, 2015
Multiple emulsions as effective platforms for controlled anti-cancer drug delivery
Ewa Dluska1, Agnieszka Markowska-Radomska1, Agata Metera1
1Faculty of Chemical & Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland.
Aim:
Developing pH-responsive multiple emulsion platforms for effective glioblastoma multiforme therapy with reduced toxicity, a drug release study and modeling.
Materials & Methods:
Cancer cell line: U87 MG, multiple emulsions with pH-responsive biopolymer and encapsulated doxorubicin (DOX); preparation of multiple emulsions in a Couette-Taylor flow biocontactor, in vitro release study of DOX (fluorescence intensity analysis), in vitro cytotoxicity study (alamarBlue cell viability assay) and numerical simulation of DOX release rates.
Results:
The multiple emulsions offered a high DOX encapsulation efficiency (97.4 ± 1%) and pH modulated release rates of a drug. Multiple emulsions with a low concentration of DOX (0.02 μM) exhibited broadly advanced cell (U87 MG) cytotoxicity than free DOX solution used at the same concentration.
Conclusion:
Emulsion platforms could be explored for potential delivery of chemotherapeutics in glioblastoma multiforme therapy.
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