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Autophagy-monitoring and autophagy-deficient mice
Akiko Kuma1,2, Masaaki Komatsu3, Noboru Mizushima1
1a Department of Biochemistry and Molecular Biology , Graduate School and Faculty of Medicine, The University of Tokyo , Tokyo , Japan.
Abstract:
Discovery of yeast autophagy-related (ATG) genes and subsequent identification of their homologs in other organisms have enabled researchers to investigate physiological functions of macroautophagy/autophagy using genetic techniques. Specific identification of autophagy-related structures is important to evaluate autophagic activity, and specific ablation of autophagy-related genes is a critical means to determine the requirements of autophagy. Here, we review currently available mouse models, particularly focusing on autophagy (and mitophagy) indicator models and systemic autophagy-related gene-knockout mouse models.