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Related Experiment Videos

Sources to variability in circulating human miRNA signatures.

Andreas Keller1, Trine Rounge2, Christina Backes1

  • 1a Chair for Clinical Bioinformatics , Saarland University , Saarbrücken , Germany.

RNA Biology
|August 19, 2017
PubMed
Summary

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Circulating microRNAs (miRNAs) show promise as disease biomarkers. This study identified 135 stable miRNAs in healthy individuals, demonstrating low variability across time, age, and sample storage, making them ideal biomarker candidates.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Circulating microRNAs (miRNAs) are increasingly recognized as potential biomarkers for various human diseases.
  • Understanding the variability of miRNAs in healthy individuals is crucial for their validation as biomarkers.

Purpose of the Study:

  • To assess the longitudinal variability of circulating miRNAs in healthy individuals.
  • To identify stable miRNA candidates for biomarker development, considering age, storage time, and sampling procedures.

Main Methods:

  • Analysis of longitudinal miRNomes from 90 serum samples stored for 23-40 years.
  • Microarray profiling of 3 serum samples per individual, collected approximately 5 years apart.
  • Statistical filtering to identify miRNAs with low inter-individual and longitudinal variability.
Keywords:
circulating biomarkerscontrol signaturesmiRNAserum

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Main Results:

  • A significant portion of the miRNome was influenced by donor age and, to a lesser extent, storage time.
  • 135 miRNAs exhibited low variability across individuals, time, and storage conditions, independent of sampling procedures.
  • 168 miRNAs showed time-dependent abundance, 56 were differentially abundant between individuals, and 169 depended on sampling procedures.

Conclusions:

  • Despite variability influenced by age and storage, a core set of 135 miRNAs presents a highly stable profile in healthy individuals.
  • These stable miRNAs are promising candidates for reliable biomarker development across diverse clinical applications.
  • Further research should focus on these stable miRNAs for accurate disease detection and monitoring.