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A Self-Assembling Peptide Gel as a Vitreous Substitute: A Rabbit Study.

Koji Uesugi^1,2, Hirokazu Sakaguchi^1,3, Yasutaka Hayashida¹

¹Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Japan.

Investigative Ophthalmology & Visual Science

|August 19, 2017

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This study shows a self-assembling peptide gel is a promising vitreous substitute. The gel demonstrated good biocompatibility and physical properties, with no observed toxicity in rabbit eyes.

Area of Science:

Biomaterials science
Ophthalmology
Regenerative medicine

Background:

The vitreous humor is essential for maintaining the eye's structure and function.
Current vitreous substitutes have limitations, necessitating the development of improved alternatives.

Purpose of the Study:

To evaluate the potential of a self-assembling peptide gel (PanaceaGel SPG-178) as a vitreous substitute.
To assess the physical properties, biocompatibility, and safety of the peptide gel in an animal model.

Main Methods:

The peptide gel was prepared at a 0.1% concentration and its physical properties (refractive index, light transmission, rheology) were analyzed.
Biocompatibility was assessed using cell viability and proliferation assays.
A pars plana vitrectomy was performed on rabbit eyes, and the gel was injected into the vitreous cavity. Control groups received balanced salt solution (BSS).

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Toxicity was evaluated through electroretinography and histological analysis post-injection.

Main Results:

The peptide gel exhibited physical properties similar to human vitreous.
No significant toxicity was observed in cellular assays or in vivo.
The gel remained transparent and stable within the vitreous cavity for 3 months without fragmentation.
Electroretinography and histological findings confirmed the gel's biocompatibility.

Conclusions:

The evaluated self-assembling peptide gel shows promise as a safe and effective vitreous substitute.
Further research may support its clinical application in ophthalmology.