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Isolation and Biophysical Study of Fruit Cuticles
Published on: March 30, 2012
Processes involving selective precipitation for the recovery of purified pectins from mango peel
Andreas Nagel1, Carina Winkler1, Reinhold Carle2
1Institute of Food Science and Biotechnology, Chair of Plant Foodstuff Technology and Analysis, Hohenheim University, Garbenstrasse 25, 70599 Stuttgart, Germany.
Abstract:
Three methods for the recovery of purified pectins from directly dried mango peel were developed, using selective precipitation of mango pectin in propan-2-ol (IPA) of adequate volume concentrations for purification. Yields, composition, macromolecular and gelling properties of the resultant pectins were compared. Effluent analyses proved postextractive removal of fruit exudate arabinogalactans. The recovery processes involved (A) washing of raw-pectin powder in IPA of defined volume concentration, (B) fractional alcoholic precipitation of dissolved raw pectin, or (C) selective pectin precipitation from the hot-acid extract of mango peel in adequately diluted IPA. High galacturonic acid contents (≥ 721g/kg) and intrinsic viscosities (≥ 320mL/g) enabled ∼2.2-fold gelling capacities compared to raw mango pectin, which resulted from the standard procedure mimicking industrial pectin recovery from established sources. Removal of the predominant impurities (coextractable exudate arabinogalactans, ash) diminished the yields to ∼49% of the raw-pectin yield. Technical feasibility of the proposed procedures was discussed.

