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Updated: Feb 24, 2026

Measurements of CO2 Fluxes at Non-Ideal Eddy Covariance Sites
Published on: June 24, 2019
Estimation of Photorespiratory Fluxes by Gas Exchange
Florian A Busch1, Ross M Deans2, Meisha-Marika Holloway-Phillips2
1Research School of Biology, The Australian National University, Acton, Canberra, ACT, 2601, Australia. florian.busch@anu.edu.au.
Abstract:
Photorespiratory fluxes can be easily estimated by photosynthetic gas exchange using an infrared gas analyzer and applying the Farquhar, von Caemmerer, and Berry (Farquhar et al. Planta 149:78-90, 1980) photosynthesis model. For a more direct measurement of photorespiratory CO2 release from glycine decarboxylation, infrared gas analysis can be coupled to membrane-inlet mass spectrometry, capable of separating the total CO2 concentration into its 12CO2 and 13CO2 components in a continuous online fashion. This chapter discusses how to calculate rates of photorespiration from Rubisco kinetics and describes in detail a method for measuring the CO2 release from glycine decarboxylation using 13CO2.
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