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Measuring In Vitro ATPase Activity for Enzymatic Characterization
Published on: August 23, 2016
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Measurement of Enzyme Activities
1Plant Physiology Department, University of Rostock, 18059, Rostock, Germany. hermann.bauwe@uni-rostock.de.
Methods in Molecular Biology (Clifton, N.J.)
|August 20, 2017
Summary
This study details methods for measuring enzyme activities in plant tissues. These protocols are crucial for understanding metabolic pathways and genetic engineering efforts.
Area of Science:
- Biochemistry
- Plant Physiology
- Molecular Biology
Background:
- Enzyme activity determination in extracts is vital for metabolic network analysis.
- It aids in evaluating enzyme-targeted genetic engineering outcomes.
- Enzyme kinetics and effector molecule impacts can be elucidated.
Purpose of the Study:
- To provide protocols for assessing enzyme activities within the photorespiratory pathway.
- To cover enzymes from 2-phosphoglycolate phosphatase to glycerate 3-kinase.
Main Methods:
- Detailed protocols for enzyme activity assays in organ/organellar extracts.
- Methods focus on the core photorespiratory pathway enzymes.
Main Results:
- Established procedures for quantifying specific enzyme activities.
- Demonstrated applicability to key photorespiratory enzymes.
Conclusions:
- The provided protocols facilitate the study of metabolic networks.
- These methods support research in plant metabolism and genetic engineering.
Keywords:
2-Phosphoglycolate phosphataseEnzyme assaysGlycerate 3-kinaseGlycine decarboxylaseGlycolate oxidaseGlyoxylate aminotransferasePhotorespirationSerine hydroxymethyltransferaseMore Related Videos
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