Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.7K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.7K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.9K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synchrotron-Based Deep Learning Network of the Inner Ear: Development and Expert Validation.

The Laryngoscope·2026
Same author

The cochlear morphometry compendium: High-resolution synchrotron measurements and normative reference values.

Journal of anatomy·2026
Same author

The tympanic covering layer contributes to basilar membrane elasticity potentially influencing human frequency resolution and speech perception.

Journal of anatomy·2026
Same author

Finite Element Modeling of Cochlear Mechanics: A Systematic Review.

Annals of biomedical engineering·2025
Same author

Statistical shape modeling of the human inner ear through micro-computed tomography imaging.

Anatomical record (Hoboken, N.J. : 2007)·2025
Same author

The Greenwood function shows close alignment with pitch perceived by cochlear implant patients with long, flexible electrode arrays and fine-structure stimulation.

Frontiers in neuroscience·2025

Related Experiment Video

Updated: Feb 24, 2026

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.7K

Improved middle-ear soft-tissue visualization using synchrotron radiation phase-contrast imaging.

Mai Elfarnawany1, Seyed Alireza Rohani2, Soroush Ghomashchi3

  • 1Department of Otolaryngology-Head and Neck Surgery, Western University, London, ON, Canada.

Hearing Research
|August 20, 2017
PubMed
Summary
This summary is machine-generated.

Synchrotron radiation phase-contrast imaging (SR-PCI) offers superior soft-tissue visualization for middle-ear finite-element modeling compared to conventional micro-CT. This advanced imaging technique simplifies sample preparation and enhances biomechanical modeling accuracy.

Keywords:
Image segmentationMicro-computed tomographyMiddle earPhase-contrast imagingSoft tissueSynchrotron radiation

More Related Videos

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

10.3K
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.5K

Related Experiment Videos

Last Updated: Feb 24, 2026

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.7K
3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

10.3K
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.5K

Area of Science:

  • Biomedical Engineering
  • Medical Imaging
  • Anatomy

Background:

  • Finite-element modeling of middle-ear structures requires high-resolution imaging.
  • Conventional techniques like micro-CT often need extensive sample preparation and contrast agents for soft-tissue visualization.

Purpose of the Study:

  • To compare synchrotron radiation phase-contrast imaging (SR-PCI) with conventional micro-computed tomography (micro-CT) for imaging unstained human temporal bones.
  • To evaluate the effectiveness of SR-PCI in improving soft-tissue contrast and accuracy for finite-element modeling of middle-ear structures.

Main Methods:

  • Four human temporal bones were imaged using both SR-PCI and conventional absorption-contrast micro-CT.
  • Images were analyzed for structural detail and soft-tissue contrast using intensity profiles and histograms.
  • Three-dimensional models of ossicles were created using semi-automatic segmentation for quantitative comparison.

Main Results:

  • SR-PCI demonstrated superior visualization of soft-tissue microstructures compared to conventional micro-CT.
  • Intensity profiles confirmed improved contrast and detectability of soft tissues with SR-PCI.
  • 3D reconstructions from SR-PCI data yielded accurate ossicle volumes comparable to micro-CT and literature values.

Conclusions:

  • SR-PCI provides enhanced visualization and accuracy for middle-ear structure modeling, including soft tissues and bone.
  • The simplified sample preparation and improved imaging quality make SR-PCI a promising tool for biomechanical studies.
  • Accessible 3D models of middle-ear structures were shared to benefit the research community.