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Updated: Feb 24, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Single Versus Dual Platform Analysis for Hematopoietic Stem Cell Enumeration Using ISHAGE Protocol.

Rahul Naithani¹, Nitin Dayal², Gaurav Dixit¹

¹Division of Hematology and Bone Marrow Transplantation, Max Superspeciality Hospital, New Delhi, India.

Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion

|August 22, 2017

Summary

This summary is machine-generated.

Accurate enumeration of CD34+ cells is crucial for hematopoietic stem cell transplantation success. Both single and dual platform analyses effectively quantify these cells, offering comparable results with distinct advantages in ease of use and cost-effectiveness.

Keywords:

Bone marrow transplantation Enumeration Flowcytometry Hematopoietic stem cells ISHAGE

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Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Published on: May 8, 2018

Area of Science:

Hematology
Immunology
Cell Biology

Background:

Hematopoietic stem cell transplantation (HSCT) offers curative potential for various hematologic and oncologic conditions.
Successful engraftment following HSCT is critically dependent on the administered dose of hematopoietic stem cells.
Accurate quantification of CD34+ progenitor cells is essential for predicting and ensuring successful engraftment.

Purpose of the Study:

To compare the efficacy and practical utility of single-platform versus dual-platform methods for enumerating CD34+ cells.
To evaluate the correlation and agreement between these two distinct analytical approaches.
To provide data to inform the selection of optimal CD34+ cell enumeration techniques in clinical settings.

Main Methods:

Analysis of 147 patient samples using both single-platform and dual-platform flow cytometry methods.
Statistical evaluation of linear correlation and agreement between the two enumeration techniques.
Assessment of practical considerations including ease of performance and cost-effectiveness.

Main Results:

A strong linear correlation was observed between CD34+ cell counts obtained by single-platform and dual-platform analyses.
Both enumeration methods demonstrated comparable effectiveness in quantifying CD34+ cells.
Single-platform analysis was found to be simpler to perform, while dual-platform analysis offered potential cost reductions.

Conclusions:

Both single and dual platform methods are reliable for CD34+ cell enumeration in HSCT.
The choice between single and dual platform analysis may depend on laboratory resources, prioritizing ease of use or cost-efficiency.
Standardized CD34+ cell quantification is vital for optimizing HSCT outcomes.