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Related Concept Videos

Methods of Classification and Identification01:28

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Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
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PathoBacTyper: A Web Server for Pathogenic Bacteria Identification and Molecular Genotyping.

Ming-Hsin Tsai1,2, Yen-Yi Liu1, Von-Wun Soo2

  • 1Institute of Population Health Sciences, National Health Research InstitutesMiaoli County, Taiwan.

Frontiers in Microbiology
|August 22, 2017
PubMed
Summary

PathoBacTyper is a new online service for identifying and genotyping pathogenic bacteria using whole-genome single nucleotide polymorphism (wgSNP) analysis. This tool aids public health and clinical labs in epidemiological surveillance and clinical investigations.

Keywords:
bacterial identificationmolecular typingnext-generation sequencingpan-genome databasewhole genome multilocus sequence typing

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Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • Next-generation sequencing (NGS) has decreased costs, enabling widespread use of bacterial whole-genome sequencing in public health and clinical settings.
  • Whole-genome single nucleotide polymorphism (wgSNP) analysis is a suitable genotyping method for epidemiological and clinical investigations in the NGS era.

Purpose of the Study:

  • To introduce PathoBacTyper, an online service for pathogenic bacteria identification and genotyping.
  • To demonstrate the utility of wgSNP analysis for bacterial typing and surveillance.

Main Methods:

  • Development of an online service, PathoBacTyper, utilizing wgSNP analysis.
  • Testing the service with diverse bacterial datasets, including Salmonella Heidelberg, Salmonella Typhimurium, Enterococcus faecium, and Legionella pneumophila isolates.

Main Results:

  • PathoBacTyper can identify and genotype over 400 pathogenic bacteria species.
  • The service demonstrated applicability for epidemiological typing and clinical investigation using real-world isolate data.

Conclusions:

  • PathoBacTyper provides a valuable resource for bacterial identification and genotyping.
  • The wgSNP-based service supports public health and clinical laboratories in surveillance and investigation efforts.