Related Experiment Video

Updated: Feb 24, 2026

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

4.0K

Antimicrobial activity of octenidine against multidrug-resistant Gram-negative pathogens

R Alvarez-Marin1,2,3,4, M Aires-de-Sousa5, P Nordmann1,2,3,6

  • 1Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, rue Albert Gockel 3, 1700, Fribourg, Switzerland.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|August 22, 2017
PubMed

Insights

Octenidine dihydrochloride (OCT) effectively kills multidrug-resistant Gram-negative pathogens in vitro. This antiseptic shows potent bactericidal activity against emerging resistant strains, aiding infection control.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Multidrug-resistant Gram-negative (MRGN) pathogens present a significant global health challenge.
  • Limited systemic antibiotic options necessitate novel infection control strategies.
  • Octenidine dihydrochloride (OCT) is a well-tolerated antiseptic with potential for MRGN dissemination control.

Purpose of the Study:

  • To evaluate the bactericidal efficacy of OCT against international epidemic clones of MRGN.
  • To assess OCT's effectiveness under varying organic loads simulating clinical conditions.
  • To determine the optimal concentration and contact time for OCT's antimicrobial action.

Main Methods:

  • Testing OCT against five key MRGN species (E. coli, K. pneumoniae, E. cloacae, A. baumannii, P. aeruginosa) according to EN13727.
  • Evaluating efficacy under 'clean' (low organic load) and 'dirty' (high organic load) conditions.
  • Assessing bactericidal activity with 0.01% and 0.05% OCT concentrations and a 1-minute contact time.

Main Results:

  • OCT demonstrated complete eradication (>5 log10 reduction) of all tested MRGN isolates within 1 minute.
  • Efficacy was consistent across different species and MRGN resistance profiles (3MRGN and 4MRGN).
  • Growth kinetics confirmed OCT's time-dependent bactericidal activity against wild-type strains.

Conclusions:

  • OCT exhibits potent bactericidal activity against multidrug-resistant Gram-negative pathogens.
  • Its effectiveness under diverse conditions suggests utility in reducing nosocomial infections.
  • OCT represents a promising tool for combating emerging highly resistant bacterial threats.

More Related Videos

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method12:03

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

27.8K
Author Spotlight: Exploring the Antibacterial Effects of Zinc Oxide Nanoparticles in Overcoming Antibiotic Resistance06:42

Author Spotlight: Exploring the Antibacterial Effects of Zinc Oxide Nanoparticles in Overcoming Antibiotic Resistance

Published on: September 27, 2024

3.0K

Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
1.2K
Antimicrobial Proteins01:23

Antimicrobial Proteins

Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
14.7K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.6K
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
7.0K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
762
Hand hygiene01:23

Hand hygiene

Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
6.2K