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Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
Published on: May 30, 2012
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JMJD1C Ensures Mouse Embryonic Stem Cell Self-Renewal and Somatic Cell Reprogramming through Controlling MicroRNA
Feng Xiao1, Bing Liao2, Jing Hu2
1Key Laboratory of Stem Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.
Stem Cell Reports
|August 23, 2017
Summary
Histone demethylase JMJD1C is crucial for maintaining embryonic stem cell pluripotency by suppressing differentiation pathways. It also aids in generating induced pluripotent stem cells through microRNA regulation.
Area of Science:
- Epigenetics
- Stem Cell Biology
- Molecular Biology
Background:
- Histone demethylases (HDMs) play roles in pluripotency, but their functions are not fully understood.
- Embryonic stem cell (ESC) self-renewal and induced pluripotent stem cell (iPSC) generation are critical processes in regenerative medicine.
Purpose of the Study:
- To investigate the role of JmjC-domain-containing protein 1c (JMJD1C), an H3K9 demethylase, in ESC self-renewal and iPSC generation.
- To elucidate the molecular mechanisms by which JMJD1C regulates pluripotency and differentiation.
Main Methods:
- Depletion of Jmjd1c in mouse ESCs.
- Analysis of ERK/MAPK signaling and epithelial-to-mesenchymal transition (EMT) markers.
- Inhibition of ERK/MAPK signaling.
- Assessment of microRNA expression (miR-200 family and miR-290/295 cluster).
- Evaluation of iPSC generation and maintenance.
Main Results:
- JMJD1C depletion induced ESC differentiation by activating ERK/MAPK signaling and EMT.
- Inhibiting ERK/MAPK signaling rescued the differentiation phenotype.
- JMJD1C, with KLF4, maintains ESC identity by regulating microRNAs to suppress ERK/MAPK signaling and EMT.
- JMJD1C is essential for efficient iPSC generation and maintenance via microRNA control.
Conclusions:
- JMJD1C is a key epigenetic regulator essential for maintaining ESC pluripotency and facilitating somatic cell reprogramming.
- JMJD1C integrates epigenetic and transcriptional control, involving microRNAs, to regulate stem cell fate.
- Targeting JMJD1C may offer therapeutic strategies for stem cell applications and regenerative medicine.
Keywords:
EMTERK/MAPK signalingH3K9 demethylaseJMJD1CKLF4embryonic stem cellsmicroRNAsreprogrammingself-renewalMore Related Videos
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