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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Feb 24, 2026

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

An integrated method for cell isolation and migration on a chip.

Xiaoqing Lv^1,2, Zhaoxin Geng³, Zhiyuan Fan^1,2

¹State Key Laboratory of Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China.

Scientific Reports

|August 23, 2017

Summary

This summary is machine-generated.

This study introduces a novel microfluidic chip for isolating cancer cells and studying their migration. The device effectively separates MCF-7 cells and assesses the impact of TGF-β on their movement and protein expression.

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Published on: February 21, 2014

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Last Updated: Feb 24, 2026

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Study of Cell Migration in Microfabricated Channels

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

Area of Science:

Biotechnology
Cancer Research
Microfluidics

Background:

Tumor cell migration is crucial for metastasis.
Magnetic manipulation offers a promising approach for cell guidance and patterning.
Existing methods for cell isolation and migration assays can be complex.

Purpose of the Study:

To develop a miniaturized microfluidic chip for simultaneous cell isolation and migration assay.
To investigate the effects of TGF-β on MCF-7 cell migration and protein expression.
To provide a novel, cost-effective tool for cancer metastasis research.

Main Methods:

Fabrication of a microfluidic chip with a magnet adapter, PDMS chip, and magnetic rings.
Isolation of MCF-7 cells from MDA-MB-231-RFP cells using anti-EpCAM antibody-coated magnetic beads.
Evaluation of TGF-β effects on MCF-7 cell migration distance and protein expression within the microfluidic chip.

Main Results:

Successful isolation of MCF-7 cells from a mixed population using magnetic beads and the microfluidic chip.
Demonstrated ability to assess cell viability during microfluidic culture.
Quantified the impact of TGF-β on MCF-7 cell migration and protein expression.

Conclusions:

The integrated microfluidic chip provides a novel, low-cost, and user-friendly platform for cell isolation and migration assays.
This method holds potential for advancing microfluidic applications in cancer metastasis research.
The developed technique offers a new avenue for biological experimentation in cell migration studies.