Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

8.2K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Accuracy of Three Different Strategies for Virtual Interocclusal Registration: An In Vitro Study.

Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.]·2026
Same author

Using Polysialylated Streptavidin as an Analytical Tool to Visualize Interaction Partners of Polysialic Acid.

Molecules (Basel, Switzerland)·2026
Same author

Polysialic Acid Synthesis on the <i>N</i>- and <i>O</i>‑Glycans of Salmonid Polysialyltransferase ST8Sia IV.

ACS omega·2026
Same author

In planta engineering of polysialylated glycoproteins using salmonid polysialyltransferases.

Scientific reports·2026
Same author

Characterization of Multilayer Structure-Graded Dental Zirconias.

Bioengineering (Basel, Switzerland)·2026
Same author

Impact of scanning errors on the trueness of digital implant impressions.

International journal of implant dentistry·2026
Same journal

Foramen rotundum versus canal of the maxillary verve.

Surgical and radiologic anatomy : SRA·2026
Same journal

Segmental vertebral artery agenesis with deep cervical reconstitution in a cervicothoracic variant cluster.

Surgical and radiologic anatomy : SRA·2026
Same journal

Influence of retrocalcaneal bursal effusion on the mechanical behavior of the Achilles tendon insertion during ankle dorsiflexion: a cadaveric study.

Surgical and radiologic anatomy : SRA·2026
Same journal

Anatomic variations in basilar artery termination: a systematic review and meta-analysis with presentation of an illustrative case.

Surgical and radiologic anatomy : SRA·2026
Same journal

Letter to the Editor "Aberrant right posterior hepatic artery originating from the gastroduodenal artery with a concomitant replaced left hepatic artery: a rare clinical variant".

Surgical and radiologic anatomy : SRA·2026
Same journal

MRI-based normative orbital anthropometric measurements in pediatric populations: age-related variations and clinical implications.

Surgical and radiologic anatomy : SRA·2026
See all related articles

Related Experiment Video

Updated: Feb 24, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
06:47

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

2.6K

Does formalin fixation influence MSCT/CBCT accuracy?

Anna Seidel1, Bastian Bergauer2, Michael Lell3

  • 1Department of Prosthodontics, Erlangen University Hospital, Glueckstraße 11, 91054, Erlangen, Germany.

Surgical and Radiologic Anatomy : SRA
|August 23, 2017
PubMed
Summary
This summary is machine-generated.

Formalin fixation does not significantly impact the accuracy of 3D bone models created using multi-slice computed tomography (MSCT) and cone beam computed tomography (CBCT). This finding supports the use of fixed specimens in future 3D imaging research.

Keywords:
3D modelCBCTDigital imagingFixationFormaldehydeMSCT

More Related Videos

Assessing Cortical Cerebral Microinfarcts on High Resolution MR Images
08:39

Assessing Cortical Cerebral Microinfarcts on High Resolution MR Images

Published on: November 20, 2015

14.0K
A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
07:10

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Published on: June 12, 2020

5.6K

Related Experiment Videos

Last Updated: Feb 24, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
06:47

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

2.6K
Assessing Cortical Cerebral Microinfarcts on High Resolution MR Images
08:39

Assessing Cortical Cerebral Microinfarcts on High Resolution MR Images

Published on: November 20, 2015

14.0K
A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
07:10

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Published on: June 12, 2020

5.6K

Area of Science:

  • Radiological imaging
  • Medical diagnostics
  • 3D modeling

Background:

  • Advanced imaging techniques like MSCT and CBCT are crucial for diagnostic medicine.
  • Assessing image accuracy and potential distortion is vital in radiological research.
  • The effect of specimen preservation methods on imaging accuracy requires investigation.

Purpose of the Study:

  • To evaluate the impact of formalin fixation on the accuracy of virtual 3D bone models.
  • To compare the accuracy of models generated using CBCT and MSCT.
  • To utilize a software-based method to eliminate human measurement error.

Main Methods:

  • A human head specimen was scanned using MSCT and CBCT, both before and after formalin preservation.
  • Digital 3D models of the lower jaw were created from the scans.
  • These models were superimposed with a master model from optical scanning for accuracy assessment.

Main Results:

  • Analysis of 200 points per specimen showed minimal mean deviation (0.01 mm for MSCT).
  • Cone beam computed tomography (CBCT) showed no significant difference in mean values between fixed and unfixed states (0.4 voxel).
  • Formalin fixation did not introduce substantial inaccuracies in the generated 3D bone models.

Conclusions:

  • Formalin fixation does not significantly compromise the accuracy of 3D bone models generated by MSCT and CBCT.
  • Researchers can confidently use formalin-fixed specimens for 3D model investigations.
  • The study validates the reliability of advanced imaging techniques for preserved anatomical samples.