Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring the effect of nitrile substituent position on fluorescence quantum yield of ESIPT-based oxazoline derivatives: A TDDFT investigation.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2022
Same author

Identification of immune-associated lncRNAs as a prognostic marker for lung adenocarcinoma.

Translational cancer research·2022
Same author

Cross-Scale Synthesis of Organic High-<i>k</i> Semiconductors Based on Spiro-Gridized Nanopolymers.

Research (Washington, D.C.)·2022
Same author

Identification of a novel alphaendornavirus from Lonicera maackii.

Archives of virology·2022
Same author

Protocol for evaluation and validation of TLR8 antagonists in HEK-Blue cells via secreted embryonic alkaline phosphatase assay.

STAR protocols·2022
Same author

Potential herb-drug interaction risk of thymoquinone and phenytoin.

Chemico-biological interactions·2022

Related Experiment Video

Updated: Feb 24, 2026

Purification of High Yield Extracellular Vesicle Preparations Away from Virus
07:15

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

12.4K

Polymer-Based Purification of Extracellular Vesicles.

Peter N Brown1, Hang Yin2,3

  • 1Drug Discovery Programme, Beatson Institute for Cancer Research, Bearsden, Glasgow, G61 1BD, UK.

Methods in Molecular Biology (Clifton, N.J.)
|August 23, 2017
PubMed
Summary

Extracellular vesicles (EVs) are crucial biomarkers. This chapter details polymer-based precipitation for EV purification and quantification, addressing challenges in detecting these small particles for diagnostic applications.

Keywords:
Electron microscopyExosome productionExosome purificationExtracellular vesiclesImmunolabelingPolymer based EV precipitation

More Related Videos

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
10:46

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Published on: May 13, 2022

4.9K
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
06:59

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

10.9K

Related Experiment Videos

Last Updated: Feb 24, 2026

Purification of High Yield Extracellular Vesicle Preparations Away from Virus
07:15

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

12.4K
Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
10:46

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Published on: May 13, 2022

4.9K
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
06:59

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

10.9K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Nanotechnology

Background:

  • Extracellular vesicles (EVs), including exosomes, are released from cells and contain disease-indicative molecules.
  • EVs hold potential for diagnostic biophysical and biochemical assays.
  • Effective EV purification and quantification are essential for their diagnostic utility.

Purpose of the Study:

  • To provide an overview of extracellular vesicle (EV) purification techniques.
  • To offer detailed instructions for purifying EVs using polymer-based precipitation.
  • To address the challenges in quantifying and characterizing small EVs.

Main Methods:

  • Polymer-based precipitation for EV isolation.
  • Quantification methods for purified EVs.
  • Characterization techniques for small extracellular vesicles.

Main Results:

  • Demonstration of polymer-based precipitation as a viable method for EV purification.
  • Successful quantification of purified EVs.
  • Discussion of challenges and potential solutions for EV detection and characterization.

Conclusions:

  • Polymer-based precipitation offers a practical approach for EV purification.
  • Accurate quantification and characterization of EVs are critical for their use in diagnostics.
  • Further development of detection methods is needed for small EVs.