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Updated: Feb 24, 2026
Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
Published on: May 13, 2022
Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)
1Department of Radiology and Medical Imaging, University of Virginia, PO BOX 801339, Fontaine Research Park, Rm180, 480 Ray C Hunt Drive, Charlottesville, 22903, VA, USA.
Abstract:
Extracellular vesicles (EVs) have recently attracted substantial attention due to the potential diagnostic and therapeutic relevance. Although a variety of techniques have been used to isolate and analyze EVs, it is still far away from satisfaction. Size-exclusion chromatography (SEC), which separates subjects by size, has been widely applied in protein purification and analysis. The purpose of this chapter is to show the applications of size-exclusion high-performance liquid chromatography (HPLC) as methods for EV characterization of impurities or contaminants of small size, and thus for quality assay for the purity of the samples of EVs.
