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Updated: Feb 24, 2026
Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles
Published on: November 14, 2025
Isolation of Extracellular Vesicles from Breast Milk
1Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Suite R10, Houston, TX, 77030, USA. xwang5@houstonmethodist.org.
Abstract:
Isolation of extracellular vesicles (EVs) from breast milk enables the downstream analysis of the contents, including various proteins, lipids, and RNAs (messenger RNAs and microRNAs). Here, we present the method of centrifugation with ExoQuick™ precipitation to isolate EVs from breast milk. It has been proven to be a rapid and effective alternative compared to conventional differential centrifugation method.
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