Related Experiment Video

Updated: Feb 24, 2026

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
09:15

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

9.5K

The idiosyncratic drug-induced gene expression changes in HepG2 cells

J Jiang1, K Mathijs1, L Timmermans1

  • 1Department of Toxicogenomics, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, The Netherlands.

Data in Brief
|August 24, 2017
PubMed

Insights

Inflammation increases susceptibility to drug-induced liver injury (DILI). This study investigated molecular mechanisms by analyzing gene expression in HepG2 cells exposed to various compounds and cytokines, revealing insights into ceramide metabolism and ER stress.

Area of Science:

  • Hepatology
  • Toxicology
  • Molecular Biology

Background:

  • Inflammatory stress is linked to increased susceptibility to idiosyncratic drug-induced liver injury (DILI).
  • The precise molecular mechanisms underlying inflammation-associated idiosyncratic drug hepatotoxicity are not fully understood.

Purpose of the Study:

  • To investigate the genome-wide gene expression patterns in response to idiosyncratic and non-idiosyncratic compounds under inflammatory conditions.
  • To elucidate the molecular mechanisms of inflammation-associated idiosyncratic drug hepatotoxicity.

Main Methods:

  • HepG2 cells were treated with high and low doses of idiosyncratic and non-idiosyncratic compounds.
  • Cells were exposed in the presence or absence of a cytokine mix for 6, 12, and 24 hours.
  • Microarray analysis using Agilent 4×44K Whole Human Genome chips was performed to assess genome-wide expression patterns.

Main Results:

  • The study identified changes in gene expression related to ceramide metabolism, endoplasmic reticulum (ER) stress, apoptosis, and cell survival pathways.
  • Differential gene expression patterns were observed between idiosyncratic and non-idiosyncratic compounds, particularly under inflammatory conditions.
  • The data provide insights into the molecular pathways affected by drug exposure and inflammation.

Conclusions:

  • Inflammation significantly influences the cellular response to drug compounds, impacting pathways crucial for liver cell survival and death.
  • Understanding these molecular mechanisms is vital for predicting and preventing idiosyncratic drug-induced liver injury.
  • The findings contribute to the knowledge base of drug hepatotoxicity and provide valuable data for further research.

More Related Videos

Cell Type-specific Gene Expression Profiling in the Mouse Liver10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

8.3K
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

13.2K

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.7K
Drug toxicity: Idiosyncratic Reactions01:16

Drug toxicity: Idiosyncratic Reactions

Idiosyncratic drug reactions represent abnormal chemical responses that vary significantly among individuals, ranging from extreme sensitivity to low doses to insensitivity to high doses. These reactions often occur due to the drug's covalent binding with serum proteins, forming a foreign hapten that triggers an immunotoxicological response. The variability in drug reactions has a strong pharmacogenetic foundation, with genetic differences crucial in how individuals metabolize drugs. For...
50
Pharmacogenetics of Phase I Enzymes: Cytochrome P450 Isozymes01:28

Pharmacogenetics of Phase I Enzymes: Cytochrome P450 Isozymes

Cytochrome P450 (CYP450) enzymes are a superfamily of heme-containing monooxygenases that play a pivotal role in Phase I drug metabolism by catalyzing oxidation and reduction reactions.These enzymes transform lipophilic xenobiotics into more hydrophilic metabolites, facilitating subsequent Phase II conjugation and eventual excretion. The CYP450 family is classified into families (e.g., CYP1–CYP3) and subfamilies (e.g., CYP2A, CYP2C), based on amino acid sequence homology.CYP450...
27
Pharmacogenomics: Identification of New Drug Targets01:29

Pharmacogenomics: Identification of New Drug Targets

Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
29
Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu01:29

Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu

Genetic variations significantly influence drug response through pharmacokinetics, receptor interactions, and biologic milieu modifications. Pharmacokinetic alterations impact drug metabolism and clearance, affecting efficacy and toxicity. Variants in drug-metabolizing enzymes, such as CYP2C9 and CYP2C19, alter drug activation and elimination. For example, CYP2C9 loss-of-function variants require lower warfarin doses to prevent excessive bleeding, while CYP2C19 variants reduce clopidogrel...
26
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
30