Related Experiment Video
Updated: Feb 24, 2026
08:25
An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation
Published on: April 27, 2021
2.7K
Lipopolysaccharide suppresses IgE-mast cell-mediated reactions
1Division of Allergy and Immunology, University of Cincinnati College of Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Summary
Lipopolysaccharide (LPS) exposure suppresses IgE-mediated anaphylaxis by down-regulating mast cell FcεRI expression via IL-10. This desensitization is temporary, suggesting therapeutic potential for the LPS-TLR-4-IL-10 pathway.
Area of Science:
- Immunology
- Allergy Research
- Microbial-Host Interactions
Background:
- IgE and mast cells are central to anaphylaxis.
- Bacterial infections may influence anaphylaxis susceptibility.
Purpose of the Study:
- To investigate the impact of lipopolysaccharide (LPS) exposure on IgE-mast cell (MC) mediated reactions in mice.
Main Methods:
- Mice (WT, tlr4-/-, IL10-/-) were exposed to LPS, and cytokines were measured.
- Passive IgE-mediated anaphylaxis, MC activation, Ca2+ mobilization, and FcεRI expression were quantified.
Main Results:
- LPS exposure suppressed IgE-MC reactions in WT mice, dependent on TLR-4.
- Suppression was linked to increased IL-10, decreased FcεRI on MCs, and reduced sensitivity to IgE.
- MC desensitization was transient, with sensitivity restored within 48 hours.
Conclusions:
- LPS-induced IL-10 down-regulates MC FcεRI, desensitizing mice to IgE reactions.
- Targeting the LPS-TLR-4-IL-10 pathway could prevent adverse IgE-mediated reactions.
Related Concept Videos
01:25Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
2.0K
Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
2.0K
02:06Allergic Reactions
32.6K
Overview
32.6K
01:30Allergic Reactions: Anaphylaxis
44
Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin,...
44
01:27Allergic Drug Reactions
1.5K
Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing...
1.5K
01:10Antibody Structure
66.0K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
66.0K
01:30Hypersensitivities
7.9K
Hypersensitivity, also known as a hypersensitivity reaction or allergic reaction, is a condition where the body's immune system reacts abnormally to a foreign substance. Such substances, that cause hypersensitivity are referred to as an allergen, could be something typically harmless to most people, like pollen or certain foods.
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...
7.9K