Related Experiment Video
Updated: Feb 24, 2026
05:58
Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
Published on: March 22, 2022
4.6K
Evaluation of biological fluid analysis using the sysmex XN automatic hematology analyzer
Vicente Aguadero1, Ruth Cano-Corres1, Eugenio Berlanga1
1Department of Biochemistry, Parc Taulí Hospital Universitari. Institut d'Investigació I Innovació Parc Taulí I3PT, Universitat Autònoma de Barcelona, Sabadell, Spain.
Cytometry. Part B, Clinical Cytometry
|August 24, 2017
Summary
The Sysmex XN-1000 analyzer shows good agreement with manual methods for white blood cell (WBC) and red blood cell (RBC) counts in most body fluids, improving lab efficiency. However, discrepancies were noted in ascitic fluid analysis.
Area of Science:
- Clinical laboratory diagnostics
- Hematology
- Fluid analysis
Background:
- Manual cell counting methods for biological fluids have limitations.
- Automated hematological cytometers offer potential improvements.
- This study assesses the Sysmex XN-1000 analyzer's performance against manual techniques.
Purpose of the Study:
- To evaluate the agreement between the Sysmex XN-1000 and manual methods for cell counts (WBC, RBC) and WBC differentiation in various body fluids.
- To assess the clinical utility and efficiency of the automated method in routine laboratory work.
Main Methods:
- Analysis of 103 fluid samples: 45 ascitic (AF), 21 synovial (SF), 33 pleural (PF), and 31 cerebrospinal (CSF).
- Cell counting performed using Sysmex XN-1000 and Fuchs-Rosenthal chamber.
- Manual WBC differentiation using May Gründwald-Giemsa stain.
- Statistical analysis included Passing-Bablok regression and Kappa index.
Main Results:
- Good agreement for WBC differentiation across all fluids and WBC counts in SF and PF.
- Optimal agreement and clinical significance for WBC and RBC counts in CSF, and RBC counts in PF.
- Disagreement in AF for WBC and RBC counts, with higher Sysmex XN-1000 results.
- Reduced laboratory response time with the automated method.
Conclusions:
- Sysmex XN-1000 generally agrees with manual methods for cell counts and differentiation in body fluids, except for ascitic fluid.
- The automated analyzer demonstrates clinical significance and improved efficiency for most fluid types.
- The findings support the incorporation of the Sysmex XN-1000 into routine laboratory practice for body fluid analysis.
