Isolation and Culture of Adipose-Derived Stromal Cells from Subcutaneous Fat

Cells with mesenchymal stem cell characteristics can be isolated from human adipose tissue stroma. Relative ease of isolation in large numbers and their ability for expansion and differentiation means that they are becoming a preferred cell type for mesenchymal regenerative medicine applications. In addition to expansion and differentiation capacity, they also express valuable paracrine activities which promote tissue formation and wound healing, including pro- and anti-fibrotic mediators. Here we describe a method for routine isolation of adipose stromal cells, culture expansion, and characterization by differentiation and then production of conditioned media.