[The exclusion of nonspecific effects in testing potential anti-inflammatory agents following intraperitoneal...

The intraperitoneal (i.p.) administration of potential antiphologistics of the group of DL-2-phenylglycinalkylester hydrochlorids caused beside the inhibition of the carrageenan rat paw oedema a high increase of the glucocorticoid-induced tyrosin transaminase activity (TTA) in the liver. Using this group of compounds the exclusion of unspecific antiphlogistic effects as a consequence of peritoneal irritation and glucocorticoid liberation was studied. For comparison phenylbutazone, hydrochlorid acid and carrageenan were examined after i.p. administration. The results showed that in contrast to hydrochlorid acid and carrageenan the antiphlogistic effects of DL-2-phenylglycinalkylester hydrochlorids were not elicited by unspecific irritation. Therefore the i.p. administration can be used in testing antiinflammatory drugs, if indirect effects have been excluded in adrenalectomized animals. The determination of the TTA-activity of the liver as an indicator of glucocorticoid liberation is not sufficient for exclusion of unspecific effects in testing antiphlogistics after i.p. administration.