Related Experiment Video
Updated: Feb 24, 2026
08:19
Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
Published on: June 1, 2012
15.1K
Glucose and Lactate Miniaturized Biosensors for SECM-Based High-Spatial Resolution Analysis: A Comparative Study.
Alice Soldà1,2, Giovanni Valenti1, Massimo Marcaccio1
1Chemistry Department "Giacomo Ciamician", University of Bologna , Via Selmi 2, 40126 Bologna, Italy.
ACS Sensors
|August 25, 2017
Summary
Miniaturized enzymatic biosensors using glucose oxidase and lactate oxidase on platinum ultramicroelectrodes were developed for single-cell metabolite monitoring. These probes enable high-resolution cellular imaging and analysis via scanning electrochemical microscopy.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Cellular Metabolism
Background:
- Miniaturized enzymatic biosensors are crucial for in vitro diagnostics and single-cell analysis.
- Scanning Electrochemical Microscopy (SECM) offers high-spatial-resolution electrochemical analysis.
- Enzyme immobilization is critical for biosensor performance but challenging at microscale.
Purpose of the Study:
- To develop and characterize miniaturized glucose and lactate biosensors for in vitro diagnostic applications.
- To evaluate enzyme immobilization techniques for ultramicroelectrode-based biosensors.
- To assess the spatial resolution and cellular imaging capabilities of these biosensors using SECM.
Main Methods:
- Fabrication of glucose and lactate biosensors by immobilizing enzymes on 10 μm platinum ultramicroelectrodes.
- Investigation of various enzyme immobilization techniques and their impact on biosensor performance metrics.
- Application of SECM for 2D imaging and analysis of single MCF10A cell metabolic activity.
- Implementation of a voltage-switch approach for quantitative cellular uptake measurements.
Main Results:
- Optimized fabrication routes for sensitive and responsive enzymatic biosensors.
- Demonstrated high spatial resolution for SECM imaging of single living cells.
- Successfully monitored glucose concentrations near single cells to assess metabolic activity.
- Quantitative measurements of cellular glucose uptake were achieved.
Conclusions:
- Miniaturized enzymatic biosensors on ultramicroelectrodes are effective for high-resolution cellular analysis.
- SECM combined with enzymatic probes enables advanced in vitro functional imaging of cells.
- The developed biosensors show promise for precise monitoring of cellular metabolism and diagnostics.
