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A 3D printed microfluidic perfusion device for multicellular spheroid cultures.

Louis Jun Ye Ong1, Anik Islam, Ramanuj DasGupta

  • 1Department of Biomedical Engineering, National University of Singapore, 4, Engineering Drive 3, E4-04-10, Singapore 117583, Singapore.

Biofabrication
|August 25, 2017
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Researchers developed a 3D printed microfluidic device for culturing multicellular spheroids, improving accessibility for organ-on-chip research. This innovation supports spheroid viability and function, advancing drug efficacy studies.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • 3D Printing

Background:

  • Organ-on-chip technologies are crucial for biological research but often lack accessibility.
  • Existing 3D printed microfluidic devices struggle to support multicellular spheroid cultures.
  • Multicellular spheroids are vital for mimicking complex biological structures and cell-cell interactions.

Purpose of the Study:

  • To develop a 3D printed microfluidic device capable of immobilizing and maintaining 3D multicellular spheroids.
  • To evaluate stereolithography (SLA) and PolyJet printing for fabricating such devices.
  • To implement a pump-free perfusion system for simplified operation.

Main Methods:

  • Fabrication of microfluidic devices using stereolithography (SLA) and PolyJet printing.
  • Development of integrated cell-immobilizing microstructures within the microfluidic network.
  • Implementation of a gravity-driven, pump-free perfusion system for medium exchange.
  • Culture and assessment of patient-derived oral squamous cell carcinoma and HepG2 liver cell spheroids.

Main Results:

  • Stereolithography (SLA) demonstrated higher fidelity in fabricating microfluidic devices with cell-immobilizing structures.
  • The 3D printed device successfully immobilized and maintained the viability and functionality of 3D multicellular spheroids.
  • The pump-free perfusion system effectively supported spheroid cultures, reducing device complexity.
  • Demonstrated good cell viability and functionality in patient-derived tumor and liver spheroids.

Conclusions:

  • This study presents the first 3D printed microfluidic device for direct spheroid culture, enhancing accessibility.
  • The developed device simplifies the prototyping and operation of microfluidic spheroid culture systems.
  • This technology holds significant potential for applications in drug efficacy, metabolism, and toxicity studies.