Related Experiment Video
Updated: Feb 24, 2026
11:33
Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
2.1K
HOTHEAD-Like HTH1 is Involved in Anther Cutin Biosynthesis and is Required for Pollen Fertility in Rice
Ya Xu1, Shasha Liu1, Yaqin Liu1
1College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Plant & Cell Physiology
|August 26, 2017
Summary
Rice HTH1 gene is crucial for anther development and pollen fertility. Suppressing HTH1 disrupts cuticle formation, leading to defective anthers and aborted pollen, highlighting its role in plant reproduction.
Area of Science:
- Plant reproductive biology
- Plant lipid biosynthesis
- Molecular genetics
Background:
- The anther cuticle is vital for male reproductive development in plants.
- The precise mechanisms of lipidic polymer synthesis in the cuticle are not fully understood.
- Arabidopsis thaliana HOTHEAD (HTH) is a candidate enzyme in long-chain fatty acid biosynthesis.
Purpose of the Study:
- To investigate the function of the rice anther-specific gene HTH1.
- To determine HTH1's role in cutin biosynthesis and its necessity for anther development and pollen fertility.
Main Methods:
- Gene expression analysis using quantitative real-time PCR (qRT-PCR) and in situ hybridization.
- Gene function analysis via CRISPR and RNA interference (RNAi) mediated gene suppression.
- Analysis of cuticle structure, pollen morphology, and fatty acid composition in anthers.
Main Results:
- HTH1 is highly expressed in rice anther epidermal cells.
- Suppression of HTH1 resulted in defective anther walls, pollen abortion, disorganized cuticles, and shriveled pollen.
- HTH1 suppression significantly reduced long-chain fatty acids and cutin monomers in rice anthers.
Conclusions:
- Rice HTH1 is essential for cutin biosynthesis.
- HTH1 plays a critical role in rice anther development and pollen fertility.
More Related Videos
Related Concept Videos
01:25Cell Signaling in Plants
6.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.7K
02:22Introduction to Plant Diversity
49.3K
From Water to Land
49.3K
02:16Light Acquisition
9.7K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
9.7K