Related Experiment Video
Updated: Feb 24, 2026
Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation
Published on: February 26, 2013
Evaluation of a Novel Cryoprobe for Atrial Ablation in a Chronic Ovine Model
Matthew R Schill1, Spencer J Melby1, Molly Speltz2
1Division of Cardiothoracic Surgery, Washington University School of Medicine, St. Louis, Missouri.
Purpose:
Cryoablation is used in the treatment of atrial fibrillation and other cardiac arrhythmias. This study evaluated a novel 10-cm flexible nitrous oxide cryoprobe in an ovine model of atrial ablation.
Description:
Six sheep were anesthetized, underwent a left thoracotomy, and were placed on cardiopulmonary bypass. A left atriotomy was performed, and the cryoprobe was applied endocardially for 120 seconds at less than -40°C to 4 sites on the left atrium. The atrium was closed and the animals were allowed to recover. After 30 days, the animals were euthanized. Transmurality was evaluated in 5-mm sections of each lesion using 2,3,5-triphenyltetrazolium chloride (TTC) and Masson's trichrome staining.
Evaluation:
All animals survived. One hundred four of 106 sections (98%) were transmural by TTC; 103 of 106 (97%) sections were transmural by trichrome staining. There was no late atrial perforation, intraluminal thrombus, or thromboembolism.
Conclusions:
The device reliably produced transmural lesions in a chronic ovine model. Its performance was equivalent to that of other nitrous oxide cryoablation systems.
More Related Videos
06:48Three-Dimensional Echocardiographic Method for the Visualization and Assessment of Specific Parameters of the Pulmonary Veins
Published on: October 28, 2020
08:00Reduction of Iatrogenic Atrial Septal Defects with an Anterior and Inferior Transseptal Puncture Site when Operating the Cryoballoon Ablation Catheter
Published on: June 15, 2015