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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Whole-Kidney Three-Dimensional Staining with CUBIC
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CUBIC pathology: three-dimensional imaging for pathological diagnosis.

Satoshi Nojima1,2, Etsuo A Susaki3,4,5, Kyotaro Yoshida6

  • 1Department of Pathology, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.

Scientific Reports
|August 26, 2017
PubMed
Summary
This summary is machine-generated.

Three-dimensional (3D) imaging using the CUBIC pipeline enhances histopathological diagnosis by overcoming limitations of conventional microscopy. This technique improves the detection of diseases like lymph node metastasis in patient tissues.

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Area of Science:

  • Pathology
  • Medical Imaging
  • Biotechnology

Background:

  • Conventional light microscopy of H&E-stained tissues is standard for histopathological diagnosis but has limitations in observation area and dimensionality.
  • Current methods struggle with 3D visualization, hindering comprehensive analysis of tissue architecture and cellular atypia.

Purpose of the Study:

  • To apply the CUBIC tissue-clearing and 3D-imaging technique to clinical pathology.
  • To evaluate the effectiveness of CUBIC for 3D imaging of patient-derived tissues and archival samples.
  • To assess CUBIC's potential for improving diagnostic sensitivity in pathological screening.

Main Methods:

  • Application of the CUBIC pipeline for three-dimensional (3D) imaging of human lung and lymph node tissues.
  • Combination of deparaffinization with CUBIC for 3D imaging of archival paraffin-embedded tissue blocks.
  • Histopathological screening of lymph node metastasis using CUBIC for enhanced detection.

Main Results:

  • CUBIC successfully enabled 3D imaging of both normal and abnormal patient-derived lung and lymph node tissues.
  • Deparaffinization and CUBIC allowed quantitative evaluation of nuclear and structural atypia in archival malignant lymphoma.
  • CUBIC-based screening significantly improved sensitivity in detecting small metastatic carcinoma nodules in lymph nodes.

Conclusions:

  • The CUBIC pipeline is a powerful tool for 3D histopathology, applicable to clinical samples.
  • CUBIC facilitates quantitative analysis and enhances diagnostic sensitivity, particularly for detecting early-stage metastases.
  • This technique holds potential for establishing a new field of 3D histopathology in clinicopathological diagnosis.