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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Engineering CRISPR-Cpf1 crRNAs and mRNAs to maximize genome editing efficiency

Bin Li1, Weiyu Zhao1, Xiao Luo1

  • 1Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.

Nature Biomedical Engineering
|August 26, 2017
PubMed
Summary

Engineered CRISPR-Cpf1 systems enhance gene editing. Modified CRISPR RNAs and messenger RNAs significantly boost gene-cutting efficiency in human cells, enabling broader genome editing applications.

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