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Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR)

Maria Giovanna Vilia1, Marta Tocchetti1, Eleonora Fonte1

  • 1Cell signaling unit, division of experimental oncology, IRCCS San Raffaele hospital, Milano, Italy.

European Cytokine Network
|August 26, 2017
PubMed

IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.

Keywords:
IL-1R8SIGIRRTIR8Toll-like receptorinterleukin-1 receptorsplicing

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