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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

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Published on: November 13, 2017

Stacking chip for quantitative bioanalysis.

Xiaohu Zhou¹, Xuechang Zhou², Bo Zheng¹

¹Department of Chemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

|August 27, 2017

Summary

This summary is machine-generated.

This study introduces a novel "stacking chip" microdevice for quantitative bioanalysis. It enables automated nanoliter solution handling, microreactor formation, and enzymatic kinetic analysis for high-throughput applications.

Keywords:

Enzyme assay Microfluidics Microwells Multistep reaction PDMS

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Area of Science:

Biotechnology
Microfluidics
Analytical Chemistry

Background:

Quantitative bioanalysis requires precise handling of small solution volumes.
Existing microfluidic devices can be complex to fabricate and operate.
High-throughput analysis is crucial for drug discovery and diagnostics.

Purpose of the Study:

To develop a simple, automated microdevice for quantitative bioanalysis.
To demonstrate the utility of the "stacking chip" for enzymatic kinetic studies.
To enable high-throughput screening using microfluidic platforms.

Main Methods:

Fabrication of a microwell-based microdevice using degassed polydimethylsiloxane (PDMS).
Integration of passive pumping, solution dispensing, plate splitting, and plate stacking.
Measurement of alkaline phosphatase enzymatic kinetics using the Michaelis-Menten model.

Main Results:

The "stacking chip" successfully performed serial operations including dispensing, splitting, and stacking.
Nanoliter solutions were accurately dispensed into microwells via PDMS microchannels.
Enzymatic kinetics of alkaline phosphatase were successfully measured and analyzed.

Conclusions:

The "stacking chip" is a simple and effective microdevice for quantitative bioanalysis.
The device facilitates automated, high-throughput analysis of biochemical reactions.
This technology holds potential for applications in diagnostics and drug screening.