Related Experiment Video
Updated: Feb 24, 2026
Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
Published on: January 12, 2020
Exploration of the pathways and interaction network involved in bladder cancer cell line with knockdown of Opa
Xuefeng He1, Xiang Ding1, Duangai Wen1
1Department of Urology, The First Affiliated Hospital of Soochow University, China.
Objectives:
In our previous study, we displayed that knockdown of Opa interacting protein 5 (OIP5) inhibited cell growth, disturbed cell cycle and increased cell apoptosis in bladder cancer (BC) cell line. Our present study aimed to explore the underlying pathways and interaction network involved in the roles of OIP5 in BC.
Methods:
Microarray analysis was conducted to obtain mRNA expression profiling of OIP5 knockdown (shOIP5) and control (shCtrl) BC cell lines. Bioinformatics analyses were performed including differentially expressed mRNAs (DEGs) identification, protein-protein interaction network construction, biological functions of prediction and ingenuity pathways analysis (IPA). Western Blotting (WB) was subjected to validate the protein expression levels of candidate DEGs in shOIP5 BC cell line.
Results:
Respective 255 up- and 184 down-regulated DEGs were identified in shOIP5 group compared with shCtrl group. In the PPI network, CAND1 and MYC had the highest connectivity with DEGs. 439 DEGs were significantly enriched in inflammatory response, regulation of cell proliferation, Toll-like receptor signaling pathway, cytokine-cytokine receptor interaction and bladder cancer. In the disease and function enrichment, DEGs were obviously involved in cellular movement, cellular growth and proliferation, cancer, inflammatory response, cell death and survival. In the OIP5 regulatory network, CDH2, IRS1, IRAK3, ID1, TNF, IL6, ITGA6, MYC and SOD2 interacted with OIP5. The WB validation results were compatible with our bioinformatics analyses.
Conclusions:
OIP5 interaction network might function as an oncogene in BC progression based on aberrant inflammatory responses. Our study might provide valuable information for investigation of tumorigenesis mechanism in BC.
Insights
Opa interacting protein 5 (OIP5) acts as an oncogene in bladder cancer (BC) by influencing inflammatory responses and cell proliferation. Understanding its network provides insights into BC tumorigenesis.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Previous studies indicated Opa interacting protein 5 (OIP5) knockdown inhibits bladder cancer (BC) cell growth, cell cycle progression, and apoptosis.
- The precise molecular pathways and interaction networks regulated by OIP5 in BC remain largely unexplored.
Purpose of the Study:
- To elucidate the underlying molecular pathways and protein-protein interaction (PPI) network associated with OIP5's role in bladder cancer progression.
- To identify key genes and signaling pathways influenced by OIP5 knockdown in BC cells.
Main Methods:
- Microarray analysis to profile mRNA expression in OIP5-knockdown (shOIP5) versus control (shCtrl) BC cells.
- Bioinformatics analyses including differential gene expression, PPI network construction, pathway enrichment, and Ingenuity Pathway Analysis (IPA).
- Western Blotting (WB) to validate key differentially expressed genes (DEGs) at the protein level.
Main Results:
- Identified 255 upregulated and 184 downregulated DEGs in shOIP5 cells compared to shCtrl.
- CAND1 and MYC emerged as highly connected nodes in the PPI network.
- DEGs were significantly enriched in inflammatory response, cell proliferation regulation, Toll-like receptor signaling, cytokine-cytokine receptor interaction, and BC pathways.
- OIP5 directly interacted with genes including CDH2, IRS1, IRAK3, ID1, TNF, IL6, ITGA6, MYC, and SOD2.
- WB validation confirmed the bioinformatics findings.
Conclusions:
- The OIP5 interaction network likely functions as an oncogene in BC progression, potentially through aberrant inflammatory responses.
- This study offers valuable insights into the complex mechanisms of BC tumorigenesis, highlighting OIP5 as a potential therapeutic target.
Related Concept Videos
01:19Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
01:31The Intrinsic Apoptotic Pathway
01:35Cancer Cell Migration through Invadopodia
01:41Non-Canonical Wnt Signaling Pathways
02:54Canonical Wnt Signaling Pathway