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RSF1 functions as an oncogene in osteosarcoma and is regulated by XIST/miR-193a-3p axis

Dapeng Wu1, Xingguo Nie2, Chao Ma2

  • 1Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China; Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|August 28, 2017
PubMed

Insights

The XIST/miR-193a-3p/RSF1 axis promotes osteosarcoma (OS) progression. Inhibiting RSF1 or targeting this axis may offer new therapeutic strategies for OS patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • RSF1 (HBXAP) is an ATP-dependent chromatin remodeling factor implicated in tumor progression.
  • The specific role of RSF1 in osteosarcoma (OS) pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the function of RSF1 in osteosarcoma.
  • To elucidate the regulatory mechanism involving XIST, miR-193a-3p, and RSF1 in OS.

Main Methods:

  • Analysis of RSF1 expression in OS cells and tissues.
  • Assessment of RSF1 inhibition effects on OS cell proliferation and invasion.
  • Investigation of the MAPK/Erk signaling pathway.
  • Detection of RSF1 as a target of miR-193a-3p.
  • Correlation analysis of XIST, miR-193a-3p, and RSF1 in OS tissues.

Main Results:

  • RSF1 expression is upregulated in OS cells and associated with advanced clinical features and poor survival.
  • RSF1 inhibition suppresses OS cell proliferation, invasion, and inactivates the MAPK/Erk pathway.
  • RSF1 is a direct target of miR-193a-3p, which is downregulated in OS.
  • XIST acts as a competing endogenous RNA (ceRNA) to repress miR-193a-3p, thereby regulating RSF1.

Conclusions:

  • The XIST/miR-193a-3p/RSF1 regulatory axis plays a significant role in osteosarcoma progression.
  • This axis represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
MAPK/ErkOsteosarcomaRSF1XISTmiR-193a-3p

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