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Related Experiment Video

Updated: Feb 24, 2026

Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering

Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering

Published on: January 24, 2025

Comparing three treponemal tests for syphilis screening.

Sean A Buono¹, Hilary A Godwin², Nicole M Green¹

¹Department of Environmental Health Sciences, Fielding School of Public Health, University of California, Los Angeles, CA 90095; Los Angeles County Public Health Laboratories, Los Angeles County, Department of Public Health, Downey, CA 90242.

Diagnostic Microbiology and Infectious Disease

|August 29, 2017

Summary

Three high-throughput treponemal immunoassays showed comparable performance for syphilis detection. The reverse algorithm identified more positive cases than the traditional method, improving syphilis screening.

Keywords:

Algorithm Immunoassay Serodiagnosis Syphilis Treponema pallidum

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Area of Science:

Clinical diagnostics
Immunology
Microbiology

Background:

Syphilis diagnosis relies on serological testing.
High-throughput immunoassays are crucial for efficient screening.
Evaluating new diagnostic platforms is essential for public health.

Purpose of the Study:

To compare the performance and usability of three high-throughput treponemal immunoassays.
To assess the effectiveness of reverse versus traditional syphilis testing algorithms.
To evaluate the clinical utility of different syphilis screening methods.

Main Methods:

Performance evaluation of Phoenix Biotech Trep-Sure, Siemens ADVIA Centaur, and DiaSorin LIAISON assays.
Analysis of 1000 serum samples using these immunoassays.
Comparison of results with Treponema pallidum particle agglutination (TP-PA) and traditional vs. reverse algorithms.

Main Results:

All three immunoassays demonstrated high sensitivity, specificity, and agreement (98-100%) with TP-PA.
Chemiluminescence platforms offered superior batching capabilities and reduced technologist time.
The reverse algorithm identified 38 additional seropositive individuals compared to the traditional algorithm.

Conclusions:

Treponemal immunoassays are reliable for syphilis screening and confirmation.
Reverse algorithm approach enhances syphilis detection rates.
Clinical evaluation aids in resolving discordant serological results.