Related Experiment Video
Updated: Feb 24, 2026
ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
Published on: March 22, 2019
An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues
M Ryan Corces1,2, Alexandro E Trevino1,3,4, Emily G Hamilton5
1Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
Abstract:
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.
