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Author Spotlight: Rabies-Specific Antibody Isotypes Detection in Sera or Cerebral Spinal Fluid Using an IFA Test
Published on: January 19, 2024
Diagnosis of bovine sarcocystosis by immunoflourescent antibody technique
Dasmabai Banothu1, Udayakumar Manchukonda1, V C Rayulu2
1Department of Veterinary Parasitology, College of Veterinary Science, Hyderabad, Sri Venkateswara Veterinary University, Rajendranagar, Hyderabad, 500030 India.
Abstract:
Studies on sarcocystosis showed an overall sero-prevalence of 79.46% by IFAT which included 80.14% in cattle and 78.59% in buffaloes out of 716 animals screened. The antibody titers of six randomly selected positive samples from different age groups of <2, 2-5, 5-10 and >10 years old bovines ranged from 16 to 64, 32 to 256, 32 to 128 and 16 to 64 with an average titer of 32 ± 2.92, 106.6 ± 34, 74.6 ± 17 and 34.6 ± 9, respectively. Significantly, lower rates of infection were observed in the cattle of below 2 years (60%) age and an ascending rate of infection in the age groups of 2-5 years (81.33%), 5-10 years (80.52%) and above 10 years (90.9%).
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The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

