Related Experiment Video

Updated: Feb 23, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.4K

Chromatographic Characterization and Method Development for Determination of Levetiracetam in Saliva: Application to

Imad I Hamdan1, Mervat Alsous2, Amira Taher Masri3

  • 1School of Pharmacy, The University of Jordan, Amman, Jordan.

Journal of Analytical Methods in Chemistry
|August 30, 2017
PubMed

Insights

Saliva can be used for therapeutic drug monitoring of levetiracetam (LVT) in children. This study developed a simple HPLC method showing good correlation between saliva and plasma LVT levels, aiding patient compliance assessment.

Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Pediatrics

Background:

  • Levetiracetam (LVT) is a common antiepileptic drug (AED).
  • Therapeutic drug monitoring (TDM) is crucial for AEDs, especially in children.
  • Less invasive sampling methods for TDM are needed.

Purpose of the Study:

  • To develop and validate a simple, isocratic HPLC method for determining LVT in saliva.
  • To assess the correlation between salivary and plasma LVT levels for TDM.
  • To investigate patient compliance in epileptic children.

Main Methods:

  • Examined LVT retention on various stationary phases, identifying C8 as most retentive.
  • Developed a simple isocratic HPLC method using protein precipitation for saliva sample preparation.
  • Validated the developed method for LVT determination in saliva.

Main Results:

  • A reasonable correlation (R=0.9) was found between plasma and salivary LVT levels.
  • The developed HPLC method is suitable for salivary TDM of LVT.
  • A significant percentage of epileptic patients showed LVT levels below the therapeutic range.

Conclusions:

  • Saliva is a viable alternative to plasma for LVT TDM in children.
  • The developed HPLC method facilitates easier TDM and compliance monitoring.
  • Findings highlight potential issues with LVT dosing or adherence in pediatric epilepsy patients.

More Related Videos

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

1.5K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.5K

Related Concept Videos

Antiepileptic Drugs: Modulators of Neurotransmitter Release Mediated by SV2A Protein01:20

Antiepileptic Drugs: Modulators of Neurotransmitter Release Mediated by SV2A Protein

Antiepileptic drugs, such as levetiracetam (Keppra) and brivaracetam (Briviact), have emerged as crucial tools in managing epilepsy. These medications exert their therapeutic effects by targeting the synaptic vesicle protein SV2A, a transmembrane glycoprotein primarily found in the brain.
SV2A is a transmembrane glycoprotein located predominantly in the brain, modulating the release of neurotransmitters for neuronal communication. Both levetiracetam and brivaracetam exhibit a high affinity for...
1.0K
Drug Concentrations: Measurements01:23

Drug Concentrations: Measurements

Drug concentration is the quantity of a drug present in a biological sample. Measuring drug amounts in biological samples allows the clinician to understand how a drug is absorbed, distributed, metabolized, and excreted. Samples can be obtained through invasive or non-invasive methods. Invasive techniques involve surgical or parenteral interventions to gather blood, cerebrospinal fluid, or tissue biopsy. Conversely, non-invasive approaches provide samples like urine, feces, and saliva.
Plasma...
1.2K
Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
231