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Bio-catalytic nanocompartments for in situ production of glucose-6-phosphate
M Lomora1, G Gunkel-Grabole, S Mantri
1Department of Chemistry, University of Basel, Klingelbergstrasse 80, CH-4056 Basel, Switzerland. cornelia.palivan@unibas.ch.
Abstract:
Cells are sophisticated biocatalytic systems driving a complex network of biochemical reactions. A bioinspired strategy to create advanced functional systems is to design confined spaces for complex enzymatic reactions by using a combination of synthetic polymer assemblies and natural cell components. Here, we developed bio-catalytic nanocompartments that contain phosphoglucomutase protected by a biomimetic polymer membrane, which was permeabilized for reactants through insertion of an engineered α-hemolysin pore protein. These bio-catalytic nanocompartments serve for production of glucose-6-phosphate, and thus possess great potential for applications in an incomplete glycolysis, pentose phosphate pathway, or in plant biological reactions.
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