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Identification of long non-coding RNA 00312 and 00673 in human NSCLC tissues
Qian Tan1, Yalan Yu1, Nandi Li1
1Department of Laboratory Medicine, Clinical Laboratory of Medicine and Center for Gene Diagnosis, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
Abstract:
Non-small cell lung cancer (NSCLC) is a fatal disease to human health. Despite the advanced progresses in cancer therapy during the past decades, NSCLC still remains the leading cause of cancer death worldwide. The long non‑coding RNAs (lncRNAs) recently have been considered as key regulators of tumor malignant. Previous studies identified that long non‑coding RNAs, linc00312 and linc00673 are markedly associated with lung cancer. However, current understanding of the two lncRNAs involving in NSCLC remains unclear. The aim of the present study was to profile their expression and clinicopathological significance in 76 patients' NSCLC tissues compared to non‑tumor tissues using reverse transcription-quantitative polymerase chain reaction. Data have indicated that the linc00312 expression level was significantly decreased in NSCLC tissues (P<0.001), while a higher linc00673 level has been detected in the same tumor tissues (P<0.01). In addition, the low expression of linc00312 was associated with the Tumor‑Node‑Metastasis stage of NSCLC (P<0.05), whereas the high expression of linc00673 was related with the histological types of NSCLC (P<0.05). In conclusion, lncRNA 00312 and 00673 may serve as potential novel biomarkers for lung cancer early diagnosis, which may play a vital role in treatments of NSCLC.
Insights
This study reveals that long non-coding RNA 00312 is decreased while long non-coding RNA 00673 is increased in non-small cell lung cancer (NSCLC) tissues. These findings suggest their potential as biomarkers for NSCLC diagnosis and treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Non-small cell lung cancer (NSCLC) remains a leading cause of cancer mortality globally.
- Long non-coding RNAs (lncRNAs) are increasingly recognized as critical regulators in tumor development.
- The specific roles of lncRNAs, linc00312 and linc00673, in NSCLC pathogenesis require further elucidation.
Purpose of the Study:
- To investigate the expression profiles of linc00312 and linc00673 in NSCLC tissues.
- To assess the clinicopathological significance of these two lncRNAs in NSCLC patients.
Main Methods:
- Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was employed.
- Expression levels of linc00312 and linc00673 were analyzed in 76 NSCLC tissues and adjacent non-tumor tissues.
Main Results:
- A significant decrease in linc00312 expression was observed in NSCLC tissues compared to non-tumor tissues (P<0.001).
- Conversely, linc00673 expression was significantly elevated in NSCLC tissues (P<0.01).
- Low linc00312 expression correlated with advanced Tumor-Node-Metastasis (TNM) stage (P<0.05), while high linc00673 expression was associated with specific NSCLC histological types (P<0.05).
Conclusions:
- The distinct expression patterns of linc00312 and linc00673 suggest their potential as novel biomarkers for early NSCLC diagnosis.
- These lncRNAs may play significant roles in the therapeutic strategies for NSCLC.
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