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Updated: Feb 23, 2026

Expression, Purification, and Antimicrobial Activity of S100A12
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Functional expression, characterization, and application of human S100B.

Lei Wu1, Xuesi Zhou1, Zhigang Xiao1

  • 1Performance Medicine Laboratory, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.

Oncology Reports
|August 30, 2017
PubMed
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Researchers developed human S100B protein and monoclonal antibodies (mAbs) for disease research. These tools effectively detect S100B in tumors and show potential for cancer therapy by inducing apoptosis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • The EF-hand calcium-binding protein S100B is implicated in various human diseases, including cancer and neurodegenerative disorders.
  • Understanding S100B's role requires reliable tools for its detection and functional analysis.

Purpose of the Study:

  • To prepare human S100B protein and develop specific monoclonal antibodies (mAbs) for its detection and functional studies.
  • To evaluate the biological activities and diagnostic potential of the prepared S100B protein and its mAbs.

Main Methods:

  • Human S100B protein expression in E. coli and purification via anion-exchange chromatography.
  • Production and validation of monoclonal antibodies using hybridoma technology, ELISA, and Western blot.
  • Assessment of S100B's effect on cancer cell migration, invasion, and apoptosis induction.

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Last Updated: Feb 23, 2026

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Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
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Main Results:

  • Successfully prepared and validated human S100B protein and specific monoclonal antibodies with low cross-reactivity.
  • Recombinant S100B promoted migration and invasion of HeLa cells.
  • S100B mAbs effectively detected S100B expression in tumor tissues and induced apoptosis in A375 cells, with increased p53 expression.

Conclusions:

  • The developed human S100B protein and monoclonal antibodies are suitable for detecting S100B expression in human tissues.
  • These tools offer promising applications for further functional investigations and clinical diagnostics in S100B-related diseases.