Related Experiment Video
Updated: Feb 23, 2026

Biofilm Removal Using Carbon Dioxide Aerosols without Nitrogen Purge
Published on: November 6, 2016
Cleansing technique using high-velocity steam-air micromist jet spray
Koichi Fukuda1, Masayuki Ishihara1, Kaoru Murakami2
1a Division of Biomedical Engineering , Research Institute, National Defense Medical College , Tokorozawa, Saitama , Japan.
A novel high-velocity steam-air micromist jet spray (HVS-AMJS) offers effective skin cleansing without chemicals. This method removes microbes and residue while providing beneficial massage effects.
Area of Science:
- Biomedical Engineering
- Dermatology
- Microbiology
Background:
- Traditional skin cleansing methods may involve chemicals or lack efficacy in removing certain residues and microbes.
- The need for effective, non-chemical cleansing techniques is growing in both clinical and personal care settings.
Purpose of the Study:
- To propose and evaluate a high-velocity steam-air micromist jet spray (HVS-AMJS) as a novel method for skin cleansing.
- To assess the efficacy of HVS-AMJS in removing residues and reducing microbial load compared to conventional methods.
- To investigate the potential physiological effects, such as massage, of the HVS-AMJS on skin.
Main Methods:
- The HVS-AMJS system utilizes low-pressure steam mixed with compressed air (pH 6.5) sprayed at controlled temperatures (42-46°C) and flow rates (~50 mL/min).
- The spray was applied at a velocity of ≥0.34 m/s from a distance of 5-10 cm.
- Efficacy was tested by removing fluorescent lotion from wood and reducing microbial counts (coliforms, total viable counts) on wood and gauze. Oily ink removal from rat skin was also assessed.
Main Results:
- HVS-AMJS successfully removed fluorescent lotion from wood surfaces, outperforming ultrasonic cleansing and manual rubbing with tap water.
- Significant reductions in coliforms and total viable counts were observed on wood and gauze treated with HVS-AMJS.
- Oily ink was effectively removed from hairless rat skin, with temporary increases in skin temperature and blood flow noted, suggesting massage effects.
Conclusions:
- The HVS-AMJS is a promising chemical-free technology for effective skin and surface cleansing.
- This method demonstrates significant efficacy in removing both chemical residues and microbial contaminants.
- The HVS-AMJS offers dual benefits of cleansing and therapeutic massage, highlighting its potential for diverse applications.
Related Concept Videos
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Key Techniques in Microbiology

