Neostriatal cholinergic neurons receive direct synaptic inputs from dopaminergic axons

We used an electron microscopic 'mirror technique' to determine whether cholinergic neurons are in direct synaptic contact with dopaminergic axons in the rat neostriatum. Tyrosine hydroxylase-immunoreactive axons make synaptic contacts with the somata and proximal dendrites of large choline acetyltransferase-immunoreactive striatal neurons which are thought to be interneurons. This provides morphological evidence that nigrostriatal dopaminergic neurons can influence monosynaptically the striatal cholinergic neurons.