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PIM-1 mRNA expression is a potential prognostic biomarker in acute myeloid leukemia
Hui Cheng1, Chongmei Huang1, Xiaoqian Xu1
1Institute of Hematology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China.
Background:
High expression of proviral integration site for Moloney murine leukemia virus-1 (PIM-1), a serine/threonine kinase, is associated with many cancers. The main purpose of this study were to investigate that the correlation between PIM-1 mRNA levels and clinicopathologic features and its clinical significance in acute myeloid leukemia (AML).
Methods:
qRT-PCR was performed for 118 de novo AML and 20 AML complete remission patients and 15 normal individuals. All statistical analysis were performed using Graphpad Prism5 software.
Results:
We observed that expression of PIM-1 mRNA was higher in AML patients than in healthy individuals and in complete remission AML patients (P = 0.0177). Further, high PIM-1 mRNA levels were more associated with high-risk FLT3+ AML patients than the FLT3- group (P = 0.0001) and were also associated with clinical factors such as risk stratification (P = 0.0029) and vital status (P = 0.0322). Kaplan-Meier survival analysis indicated that PIM-1 mRNA expression correlated with overall survival (OS), disease free survival (DFS), and relapse rate (RR) in AML patients. Most importantly, the high PIM-1-expressing patients took longer to achieve complete remission than the low expression group (P = 0.001). In addition, the complete remission rate was significantly lower in the high PIM-1 group (P = 0.0277) after induction therapy.
Conclusions:
Above results suggest that PIM-1 mRNA levels may be an independent prognostic factor in AML.
Insights
Proviral integration site for Moloney murine leukemia virus-1 (PIM-1) mRNA levels are elevated in acute myeloid leukemia (AML) patients. High PIM-1 expression correlates with poorer prognosis and treatment outcomes in AML.
Area of Science:
- Oncology
- Molecular Biology
- Hematology
Background:
- Proviral integration site for Moloney murine leukemia virus-1 (PIM-1) is a serine/threonine kinase implicated in various cancers.
- Elevated PIM-1 expression is frequently observed in malignant conditions.
Purpose of the Study:
- To investigate the correlation between PIM-1 mRNA levels and clinicopathologic features in acute myeloid leukemia (AML).
- To determine the clinical significance of PIM-1 mRNA expression in AML patients.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was employed to measure PIM-1 mRNA levels.
- The study included 118 de novo AML patients, 20 AML complete remission patients, and 15 healthy individuals.
- Statistical analyses were conducted using Graphpad Prism5 software.
Main Results:
- PIM-1 mRNA levels were significantly higher in AML patients compared to healthy individuals and those in complete remission.
- High PIM-1 mRNA expression was associated with high-risk FLT3+ AML, adverse risk stratification, and poorer vital status.
- PIM-1 mRNA levels correlated with overall survival, disease-free survival, and relapse rates, and were linked to longer time to complete remission and lower remission rates.
Conclusions:
- PIM-1 mRNA levels demonstrate potential as an independent prognostic factor in acute myeloid leukemia.
- These findings highlight PIM-1 as a potential therapeutic target or biomarker in AML management.
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