Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

807
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
807
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.1K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.1K
Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

7.3K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
7.3K
Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

1.3K
The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
1.3K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.7K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.7K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

708
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
708

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing the impact of carbamate insecticide aminocarb on human plasma protein through biophysical and computational methods.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Detection of endogenous LINE-1 ORF2p and its potent reverse transcriptase activity in the MCF-7 breast cancer cell line.

The FEBS journal·2025
Same author

Scavenging of reactive oxygen and nitrogen species using nanoparticles and their applications in disease management.

RSC advances·2025
Same author

Exploring Dysregulated Plasma Metabolites in Sickle-Cell Disease Patients Using Comparative NMR-Based Metabolomics.

Magnetic resonance in chemistry : MRC·2025
Same author

<sup>13</sup>C and <sup>15</sup>N resonance assignments of the DNA binding domain of interferon regulatory factor-3.

Biomolecular NMR assignments·2025
Same author

Polymeric Nanodiscs Comprising 5-Fluorouracil for Inhibition of Protein Aggregation and Their Anti-Alzheimer's Activity in the <i>Drosophila</i> Model.

ACS chemical neuroscience·2024

Related Experiment Video

Updated: Feb 23, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

16.1K

Super-Resolved Nuclear Magnetic Resonance Spectroscopy.

Satish Mulleti1, Amrinder Singh2, Varsha P Brahmkhatri2

  • 1Department of Electrical Engineering, Indian Institute of Science, Bangalore, 560012, India.

Scientific Reports
|August 31, 2017
PubMed
Summary
This summary is machine-generated.

We developed a new nuclear magnetic resonance (NMR) method using finite-rate-of-innovation (FRI) sampling to overcome resolution limits. This super-resolution technique accurately analyzes overlapping or broadened NMR peaks, enabling new insights into molecular interactions.

More Related Videos

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

6.1K
High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

8.0K

Related Experiment Videos

Last Updated: Feb 23, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

16.1K
High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

6.1K
High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

8.0K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biophysics

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy is crucial for molecular analysis.
  • Overlapping or broadened peaks in NMR spectra limit resolution and hinder accurate chemical shift determination.
  • Rapidly decaying signals and large linewidths are common challenges in NMR applications.

Purpose of the Study:

  • To introduce a novel method that surpasses the resolution limitations of conventional NMR spectroscopy.
  • To enable accurate estimation of chemical shift values for challenging, overlapping, or broadened NMR peaks.
  • To facilitate the study of molecular interactions by precisely measuring chemical shift changes.

Main Methods:

  • Employed finite-rate-of-innovation (FRI) sampling principles for signal reconstruction.
  • Developed a super-resolution approach that requires fewer measurements than traditional methods.
  • Applied the novel method to analyze the formation of a Gold nanorod-protein complex.

Main Results:

  • Achieved super-resolution beyond the capabilities of current NMR techniques.
  • Successfully measured minute chemical shift variations during Gold nanorod-protein complex formation.
  • Demonstrated the ability to quantify the strength of molecular interactions through NMR analysis.

Conclusions:

  • The FRI-based NMR method effectively breaks the resolution barrier in spectroscopy.
  • This technique allows for unprecedented accuracy in analyzing complex NMR spectra.
  • Opens new avenues for accelerating multidimensional NMR applications and studying diverse molecular systems.