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Updated: Feb 23, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
Published on: May 1, 2020
Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins
Verónica Muñoz-Soriano1,2, Ana Domingo-Muelas3,2, Tianlu Li4,2,5
1Departamento de Genética, Universidad de Valencia, 46100, Burjassot, Spain.
Abstract:
Elongation factor eIF5A is required for the translation of consecutive prolines, and was shown in yeast to translate polyproline-containing Bni1, an actin-nucleating formin required for polarized growth during mating. Here we show that Drosophila eIF5A can functionally replace yeast eIF5A and is required for actin-rich cable assembly during embryonic dorsal closure (DC). Furthermore, Diaphanous, the formin involved in actin dynamics during DC, is regulated by and mediates eIF5A effects. Finally, eIF5A controls cell migration and regulates Diaphanous levels also in mammalian cells. Our results uncover an evolutionary conserved role of eIF5A regulating cytoskeleton-dependent processes through translation of formins in eukaryotes.
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