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Size-Controllable Gold Nanopores with High SERS Activity.

Hai-Ling Liu1, Jiao Cao1, Sumaira Hanif1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.

Analytical Chemistry
|August 31, 2017
PubMed
Summary
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Researchers developed novel gold nanoporous spheres (GPS) for advanced protein analysis. These GPS structures enable precise nanopore sizing and offer combined ionic current rectification and surface-enhanced Raman scattering (SERS) for enhanced detection capabilities.

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Area of Science:

  • Nanotechnology
  • Analytical Chemistry
  • Biophysics

Background:

  • Nanopore sequencing advances DNA analysis but struggles with complex protein identification.
  • Current methods for protein analysis using nanopores are limited by ionic current fluctuations.
  • There is a need for size-controllable nanopores with optical activity for enhanced protein structural information.

Purpose of the Study:

  • To develop novel nanopore structures with optical activity for improved protein sequencing.
  • To investigate the properties of self-assembled gold nanoparticles (AuNPs) for nanopore applications.
  • To explore the potential of gold nanoporous spheres (GPS) for simultaneous ionic current and SERS analysis.

Main Methods:

  • Self-assembly of 7 ± 1 nm gold nanoparticles (AuNPs) to form gold nanoporous spheres (GPS) on glass capillaries.
  • Controlled nanopore formation using different dithiol linker molecules (C3, C6, C9).
  • Characterization of nanopore size, ionic current rectification, and surface-enhanced Raman scattering (SERS) activity.

Main Results:

  • Reproducible nanopore sizes (around 5.1 nm) were achieved using varying linker lengths.
  • GPS nanostructures demonstrated high ionic current rectification even in high electrolyte concentrations (290 mM).
  • Significant SERS enhancement factors (>1 × 10^5) were observed due to nanopores and 'hot spots' within the GPS.

Conclusions:

  • The developed GPS nanostructures offer a promising platform for advanced molecular detection.
  • GPS exhibits dual functionality: ionic current rectification and SERS activity.
  • Successful application of GPS for SERS-based detection of glutathione from a single HeLa cell demonstrates its potential in biological sensing.