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Ionic Strength: Overview

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The ionic strength of a solution is a quantitative way of expressing the total electrolyte concentration of a solution. This concept was first introduced in 1921 by two American physical chemists, Gilbert N. Lewis and Merle Randall, while describing the activity coefficient of strong electrolytes. During the calculation of ionic strength (I or μ), all the cations and anions are considered. However, the concentration (c) of an ion with a greater charge number (z) has a greater contribution...

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Updated: Feb 23, 2026

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

Ionic Strength Sensing in Living Cells.

Boqun Liu¹, Bert Poolman¹, Arnold J Boersma¹

¹Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute & Zernike Institute for Advanced Materials, University of Groningen , Nijenborgh 4, 9747 AG Groningen, The Netherlands.

ACS Chemical Biology

|August 31, 2017

Summary

This summary is machine-generated.

Scientists developed novel protein sensors to measure ionic strength within living cells. This breakthrough allows real-time observation of ionic changes at the single-cell level, advancing in vivo biochemistry understanding.

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Area of Science:

Biochemistry
Cell Biology
Biophysics

Background:

Understanding intracellular ionic strength is crucial for comprehending the behavior of charged biomacromolecules in their native cellular environment.
Existing methods lack the precision and real-time capabilities to assess ionic strength within living cells.

Purpose of the Study:

To develop and validate the first protein-based sensors for quantifying ionic strength in living cells.
To enable spatiotemporal monitoring of ionic strength dynamics at the single-cell level.

Main Methods:

Protein engineering of Förster resonance energy transfer (FRET) based probes.
Utilizing FRET technology to detect changes in ionic strength.
Single-cell imaging and analysis.

Main Results:

Successful design and implementation of FRET-based protein sensors capable of measuring ionic strength.
Demonstration of the probes' ability to track ionic strength changes within individual cells over time.
Validation of the sensor's sensitivity and specificity for intracellular ionic conditions.

Conclusions:

The developed FRET protein sensors represent a significant advancement in measuring intracellular ionic strength.
This technology provides unprecedented insights into cellular biochemistry and the behavior of biomacromolecules.
Future applications include studying various cellular processes influenced by ionic strength fluctuations.