Nocardioides thalensis sp. nov., isolated from a desert

Area of Science:

• Microbiology
• Bacteriology
• Taxonomy

Background:

• Actinobacteria are crucial in various environments.
• The genus Nocardioides comprises diverse species with significant ecological roles.
• Exploring novel bacterial isolates aids in understanding microbial diversity.

Purpose of the Study:

• To isolate and characterize a novel actinobacterial strain from the Thal desert.
• To determine the taxonomic position of the novel strain using a polyphasic approach.
• To propose a new species designation based on comprehensive data.

Main Methods:

• Polyphasic taxonomy including 16S rRNA gene sequencing and DNA-DNA hybridization.
• Phenotypic characterization (Gram staining, motility, colony color, biochemical tests).
• Chemotaxonomic analyses (fatty acid profiling, cell-wall composition, polar lipids, menaquinones, DNA G+C content).

Main Results:

• Strain NCCP-696T showed 98.2% 16S rRNA gene sequence similarity to Nocardioides panacisoliGsoil 346T.
• Phenotypic and chemotaxonomic data supported its distinctness from known Nocardioides species.
• DNA-DNA hybridization value was 56.3±1.4% with N. panacisoliGsoil 346T.
• Predominant fatty acids included Iso-C16:0, C18:1ω9c, C17:1ω8c, and C17:0.
• Major menaquinone was MK-8(H4) (98.7%).
• DNA G+C content was 71.6 mol%.

Conclusions:

• Strain NCCP-696T represents a novel species within the genus Nocardioides.
• The proposed name for this new species is Nocardioides thalensis sp. nov.
• The type strain is NCCP-696T (=DSM 103833T=CCTCC AB 2016296T).