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Related Concept Videos

Rab Proteins01:14

Rab Proteins

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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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Related Experiment Video

Updated: Feb 23, 2026

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
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Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

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Large protein as a potential target for use in rabies diagnostics.

I S Santos Katz, M H Dias, I F Lima

    Acta Virologica
    |September 1, 2017
    PubMed
    Summary

    This study developed a mass spectrometry proteomics technique to identify the L protein of the rabies lyssavirus (RABV). This method offers a promising new approach for rabies diagnosis and antigen identification.

    Keywords:
    rabies virus; large protein; street virus; target; mass spectrometry; proteomics.

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    Area of Science:

    • Virology
    • Immunology
    • Proteomics

    Background:

    • Rabies is a global zoonotic viral disease posing a significant public health risk.
    • The rabies lyssavirus (RABV) genome encodes five proteins crucial for pathogenicity.
    • The L protein contains conserved regions, making it a target for diagnostic development.

    Purpose of the Study:

    • To develop a mass spectrometry (MS)-based proteomics technique for identifying the RABV L protein.
    • To evaluate MS-based proteomics as a method for rabies antigen identification.
    • To explore alternative diagnostic approaches for rabies.

    Main Methods:

    • Development of a technique for identifying the L protein of RABV using MS-based proteomics.
    • Analysis of L protein from various RABV strains across different hosts.

    Main Results:

    • Successful identification of the RABV L protein using the developed MS-based proteomics technique.
    • Demonstration of MS-based proteomics as a viable method for antigen identification.
    • Validation of the technique across different RABV strains and hosts.

    Conclusions:

    • MS-based proteomics is a potential method for identifying rabies antigens.
    • This technique offers a promising alternative for the development of new rabies diagnostic tools.
    • Further development could enhance rabies surveillance and control strategies.